Inhibition of Microsome-Mediated Binding of Benzo (Α) Pyrene to "Dna By Cytosolic Reaction From Liver And Skin Rats in Cvitro
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چکیده مقاله:
Purpose: The aim of this study was to evaluate the effect of age on the capacity of liver and epiderm of adult and weanging rats in transformation of Benzo (α) Pyrene. Materials and Methods: In a metabolic activiation assay system, cytochorome P-50 (from microsomal fraction) catalyses the formation of reactive epoxide of BaP which can then interact with exogenous DNA The capacity of cytochrome P-450 for BαP-DNA adducts formation from different organs are adult rat live>>> adult rat skin>> weanging rat liver> weanling rat skin. With the addition of cytosol (the source of glutathione S transferase (GST) to the microsome-mediated system an In vitro detoxification model has been established. Results: The results obtained by the addition of different cytosolic samples are shown in several tables in the text. These data show that isolation of subcellular fractions from young rats are less efficient in the biotransformation of BαP and detoxification by GSH is a passive process that depends on activation process. Also metabolic activation of BaP with cytochorome P-450 is not the first degree with glutathione detoxification. However, the results obtined In vitro do not reflect the exact change In vivo.
منابع مشابه
MICROSOME-MEDIATED BENZO[A]PYRENE-DNA BINDING AND INHIBITION BY CYTOSOLIC FRACTIONS FROM LIVER AND SKIN OF ADULT AND WEANLING RATS
Biotransformation of benzo[a]pyrene (BaP) in the presence of microsomal fractions derived from liver and epiderm of adult and weanling rats was examined. The aim of this study was to evaluate the effect of age on the capacity of two organs in transformation of BaP. Subcellular fractions were prepared from skin and liver by ultracentrifugation and were used as the source of BaP metabolizing enzy...
متن کاملmicrosome-mediated benzo[a]pyrene-dna binding and inhibition by cytosolic fractions from liver and skin of adult and weanling rats
biotransformation of benzo[a]pyrene (bap) in the presence of microsomal fractions derived from liver and epiderm of adult and weanling rats was examined. the aim of this study was to evaluate the effect of age on the capacity of two organs in transformation of bap. subcellular fractions were prepared from skin and liver by ultracentrifugation and were used as the source of bap metabolizing enzy...
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15 صفحه اولMetabolic activation and DNA adduct formation of Benzo(a) pyrene by adult and newborn rat skin and liver microsomes
Benzo(a) pyrene is a carcinigen polycyclic aromatic hydrocarbon which diffuses into the environment from combustion of organic meterials.based on various epidemiological evidences it is related to lung,skin and liver cancer.mutagenicity,and immunosuppressivety are among important biological effects of Benzo(a) pyrene.after absorbtion and distribution in the body,it undergoes epoxidation by cyto...
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عنوان ژورنال
دوره 1 شماره 4
صفحات 53- 62
تاریخ انتشار 2003-11
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